RNA Auto Plating Workbook Process orchestrates sample preparation from RnD database lookup through final CSV export for automated liquid handling systems. The workflow retrieves clinical sample metadata by Clinical_Sample_ID or Tube_ID, validates data integrity, and populates an Auto Plating Input sheet with batch parameters, well positions, and volume calculations. Critical decision points handle manual plating exceptions, mass output verification (39–41 ng range), RNA concentration thresholds, and flag resolution before export. Users can fork this template-driven process on Diagrams.so to customize for their lab's sample tracking system, validation rules, or plate layout requirements. The workbook enforces data quality gates—duplicate sample checks, volume sufficiency (≥25 µL), concentration ranges (2.74–3.32 ng/µL)—reducing downstream automation errors and ensuring reproducible sample preparation.